Figure 2.

Sequencing results of SMAD4 for HT29/CX1, VACO10, and CACO2. (a)i shows the mutated cell line HT29/CX1 (c.931C>T transition causing Q311X amino acid truncation) compared with (a)ii wild type. (b)i SMAD4 exon 5 of VACO10 showing the c.715C>T transition causing Q239X truncation, compared with (b)ii wild type. (c)i SMAD4 exon 8 of CACO2 showing the c.1051G>C nucleotide change, which results in a D351H missense change, compared with (c)ii wild type. Note the lack of a wild-type sequence underlying a mutated (starred) sequence. The corresponding wild-type base is shown in a box.