Table 1.
Summary of molecular and cytogenetic data for 18q21.1 status in colorectal cancer cell lines
| Cell line | MSI* | B8 Western† | SMAD4 mutation‡ | Predicted effect of mutation§ | Loss at SMAD4¶ | Loss at DCC∥ | Karyotype** | 18q21 status‡‡ | TGFBIIR†† |
|---|---|---|---|---|---|---|---|---|---|
| COLO678 | MSI− | Absent | EX1 − 11del | No protein | Yes | Yes | ? | — | |
| COLO201 | MSI− | Absent | EX1 − 4del | No protein | Yes | Yes | 78 | 2 copies | None |
| COLO205 | MSI− | Absent | EX1 − 4del | No protein | Yes | Yes | 68 | 2 copies | None |
| VACO10 | MSI− | Absent | c.715C>T (Q239X), EX5 | Nonsense | Yes | Yes | 115 | — | None |
| C10 | MSI− | Absent | IVS6 − 1G>T | Splice disruption | Yes | Yes | 49 | — | — |
| HT29/CX1 | MSI− | Absent | c.931C>T (Q311X), EX7 | Nonsense | 2/3 markers | Yes | 71 | 2 copies | None |
| SW480 | MSI− | Absent | IVS7 + 5G>C | Splice disruption | Yes | Yes | 57 | 1 copy | None |
| SW620 | MSI− | Absent | IVS7 + 5G>C | Splice disruption | Yes | Yes | 50 | 1 copy | None |
| CACO2 | MSI− | Present | c.1051G>C (D351H), EX8 | Missense | Yes | Yes | 96 | — | None |
| C80 | MSI− | Present | c.1051G>C (D351H), EX8 | Missense | Yes | Yes | 69 | 2 copies | — |
| SW403 | MSI− | Absent | EX10 − 11del | Truncated protein | Yes | Yes | 68 | 2 copies | — |
| SW948 | MSI− | Present | c.1609G>T (D537Y), EX11 | Missense | Yes | No | 67 | — | — |
| SW1222 | MSI− | Present | c.1619T>G (L540R), EX11 | Missense | Yes | Yes | ? | 18q loss | — |
| SW1116 | MSI− | Absent | None found | Yes | Yes | 63 | — | — | |
| HT55 | MSI− | Absent | None found | Yes | Yes | 80 | 2 copies | — | |
| C106 | MSI− | Absent | None found | Yes | Yes | 79 | 2 copies | — | |
| PC/JW | MSI− | Absent | None found | Yes | Yes | 70 | 2 copies | — | |
| SW1417 | MSI− | Absent | None found | Yes | No | 70 | 2 copies | — | |
| SW837 | MSI− | Present | None found | Yes | Yes | 40 | 1 copy | None | |
| C99 | MSI− | Present | None found | Yes | Yes | 52 | 1 copy | — | |
| C84 | MSI− | Present | None found | No | Yes | 56 | 1 copy | — | |
| C125 | MSI− | Present | None found | Yes | Yes | 60 | 1 copy | — | |
| VACO4A | MSI− | Present | None found | Yes | Yes | 60 | 2 copies | — | |
| HCA46 | MSI− | Present | None found | Yes | Yes | 71 | 2 copies | None | |
| C32 | MSI− | Present | None found | Yes | Yes | 74 | 2 copies | — | |
| LIM21-1863 | MSI− | Present | None found | Yes | Yes | 80 | 3 copies | — | |
| C70 | MSI− | Present | None found | Yes | Yes | 127 | 4 copies | — | |
| COLO320 | MSI− | Present | None found | Yes | Yes | 53 | — | None | |
| VACO4S | MSI− | Present | None found | Yes | Yes | 64 | — | — | |
| LS1034 | MSI− | Present | None found | Yes | Yes | 77 | — | — | |
| HRA19 | MSI− | Present | None found | Yes | Yes | ? | — | — | |
| SKC01 | MSI− | Present | None found | No | Yes | hypertriploid | — | — | |
| HCT8 | MSI+ | Present | None found | No | No | ? | — | — | |
| LS180 | MSI+ | Present | None found | No | No | 45 | — | — | |
| SW48 | MSI+ | Present | None found | No | No | 47 | — | Mutant × 2 | |
| LS174T | MSI+ | Present | None found | No | No | 45 | 2 copies | Mutant × 2 | |
| DLD1/HCT15 | MSI+ | Present | None found | No | No | 46 | 2 copies | Mutant × 2 | |
| LOVO | MSI+ | Present | None found | No | No | 49 | 2 copies | Mutant × 2 | |
| VACO5 | MSI+ | Present | None found | No | No | 47 | 2 copies | Mutant × 2 | |
| HCA7 | MSI+ | Present | None found | No | No | 43 | 2 copies | Mutant × 2 | |
| HCT116 | MSI+ | Present | None found | No | No | 45 | 2 copies | Mutant × 2 | |
| LS411 | MSI+ | Present | None found | No | No | 75 | 2 copies | Mutant × ? | |
| GP2D | MSI+ | Present | None found | No | No | 46 | 2 copies | — | |
| COLO741 | MSI+ | Present | None found | Yes | Yes | ? | 2 copies | — |
COLO201/COLO205, HT29/CX1, and DLD1/HCT8/HCT15 are essentially identical cell lines.
*
MSI status.
†
SMAD4 protein expression as assessed by using B8 and western blotting.
‡
Identified SMAD4 mutations.
§
Predicted effect of SMAD4 mutations.
¶
∥ Allelic loss as inferred from homozygosity at microsatellite markers near SMAD4 and at DCC, respectively.
**
Modal chromosome number of cell line (?, not known).
‡‡
18q21.1 status as determined by CGH, SKY, and FISH (—, not done).
††
TGFBIIR mutation status (—, not done).