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. Author manuscript; available in PMC: 2018 Jul 21.
Published in final edited form as: ACS Chem Biol. 2017 Jun 7;12(7):1883–1892. doi: 10.1021/acschembio.7b00147

Figure 3. Validation of functional relevance of WEE1 and PLK1 in H322 cells.

Figure 3

(A) Immunoblot analysis of the effects of AZD1775 treatment in comparison with the PLK1 inhibitor BI-2536 on PARP1 and caspase 3 cleavage in H322 cells at the indicated time points and concentrations. Treatment with 100 nM staurosporine (STS) served as positive control. (B) Immunoblot analysis for indicated proteins of siRNA-mediated knockdown of WEE1 and PLK1 in H322 cells after 48 h. (C) Effects of siRNA-mediated knockdown of WEE1 and PLK1 on H322 cell number after 48 h and 72 h. Asterisks indicate p-value ≤ 0.05 (*), 0.01 (**) and 0.001 (***) as determined by t-test.