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. Author manuscript; available in PMC: 2018 Jul 21.
Published in final edited form as: ACS Chem Biol. 2017 Jun 7;12(7):1883–1892. doi: 10.1021/acschembio.7b00147

Figure 6. Effects of AZD1775 on γH2AX levels in WEE1 CRISPR-Cas9 knockout cells.

Figure 6

Immunoblot analysis of γH2AX and total H2AX in parental H322 cells and indicated CRISPR-Cas9 knockout (cWEE1-KO18, cWEE1-KO19) and negative control (cWEE1) clones upon 4 h AZD1775 (1 μM) or cisplatin (17 μM) treatment. Arrows indicate un-ubiquitinated (~16 kDa) and mono-ubiquitinated (~25 kDa) γH2AX.