Figure 3.

L7-4 Works as a Purkinje Cell-Specific Promoter in the Mouse Cerebellum In Vivo

(A) Schema depicting the lentiviral vectors expressing GFP by the L7-1 or L7-4 promoter and the viral injection to the mouse cerebellum. (B–E) Representative images of native GFP fluorescence on sagittal cerebellar sections at 1 week after viral injection. (B–E) The sections expressing GFP by the L7-1 (B and C) or L7-4 (D and E) promoter are presented. (F) Graph showing the relative GFP intensity of cerebellar sections lentivirally expressing GFP by the L7-4 promoter as compared with those induced by the original L7-1 promoter (n = 4 mice; t₍₆₎ = −6.55; ***p = 0.001 by Student’s t test). Error bars indicate SEM. (G) Schema describing the method of lentivirus-based generation of a transgenic mouse expressing GFP by the L7-4 promoter. (H–J) Bright-field (H) and native GFP fluorescent (I and J) images of a sagittal section of the whole brain (H and I) and the enlarged GFP image of the cerebellar cortex (J). Scale bars, 50 μm (A–D and J) and 2 mm (H and I).