Figure 7.

The L7-6 Promoter Behaves Identically as a Purkinje Cell-Specific Promoter in Marmoset Brain

(A–C) Native GFP fluorescent image of the sagittal section of the marmoset cerebellum 5 weeks after the viral injection. A 1.9-year-old marmoset received cerebellar injection of 100 μL of AAV9 vectors expressing GFP by the mouse L7-6 promoter (1.0 × 10¹³ viral genomes/mL). Low-magnified images of GFP fluorescence alone (A) and GFP fluorescence superimposed on the bright field (B) and the enlarged GFP fluorescent image (C) were presented. (D–F) Immunohistochemical analysis of the sagittal section of the cerebellum expressing GFP under the control of the L7-6 promoter. The section was double-labeled with GFP and parvalbumin. Fluorescent images of GFP (D) and parvalbumin (E) and the merged image (F) are shown. Scale bars, 2 mm (A and B), 400 μm (C), and 50 μm (D–F).