Figure 2.

miR-122a-Mediated Suppression of Ad Gene Expression

(A) miR-122a expression levels in adult and neonatal livers. The liver was collected from neonatal and adult mice to recover total RNA. miR-122a expression levels were determined by real-time RT-PCR and normalized by expression levels of U6 snRNA. (B) The leaky expression level of Ad genes in the neonatal mouse liver following systemic administration of Ad vectors. Neonatal mice were administered Ad vectors at a dose of 5.9 × 10¹¹ IFU/kg via retro-orbital sinus. Two days after transduction, the Ad gene expression levels in the liver were determined by real-time RT-PCR. Results are shown as the averages ± SD (n = 5–6). DOL, day of life.