Figure 3.
Association of HDAC1 with PC. (A) Elution profile of PC. The silver-stained polyacrylamide gel corresponding to the portion with PC protein is shown. Column fraction is indicated. (B) Activity profile of HDAC in eluted fractions. Aliquots of indicated fractions were assayed for HDAC activity with 3H-labeled acetylated core histones in the absence or presence of TSA (100 ng/ml). Values shown are the averages of duplicate experiments. (C) Column input (1 μl) and peak fraction (5 μl) were analyzed by immunoblotting with various antibodies. Note that tagged PC was detected by an anti-FLAG antibody. (D) Coimmunoprecipitation of HDAC1 and PC from embryonic nuclear extracts. Embryonic nuclear extracts (lane 1) and immunoprecipitates of the extracts with affinity-purified HDAC1 antibody (lane 2) or preimmune serum (lane 3) were analyzed by immunoblotting with an anti-PC antibody. The positions for PC and IgG are indicated.