Figure 4.

Colocalization of PSC and HDAC1 at a bxd PRE. Polytene chromosomes from wild type (A–C) or a transgenic line carrying a bxd-14 PRE at 62A (D–F) were labeled simultaneously with mouse monoclonal PSC antibodies (A and D) and affinity-purified rabbit HDAC1 antibody (B and E). The merged images are also shown (C and F). The insertion site is indicated by the arrow. Only the tip of chromosome arm 3L, which includes 62A, is shown. The specificity of HDAC1 labeling was confirmed by negative labeling of polytene chromosomes from Hdac1^P-UTR homozygous larvae or from wild-type larvae with blocked antibody.