Table 3. Effect of DNA degradation on ddPCR readings.
Sample type | Copies/PCR upon different treatments | ||||
---|---|---|---|---|---|
(0.032 ng DNA in AE Buffer) | Replicate | No treatment | Taq I digestion | 2 min sonication | 10 min sonication |
Raw beef DNA | 1 | 6800 | 4400 | 6000 | 1380 |
2 | 6760 | 4360 | 6520 | 1480 | |
Cooked beef DNA | 1 | 2040 | 1360 | 1800 | 1520 |
2 | 1820 | 1300 | 1680 | 1600 |