Table 2. Forward genetics approach showing identification of the Tgm9 insertion sites in known soybean mutants identified from the progenies of mutable line T322 (w4-m).
| Name of line |
Mutant |
Insertion site |
Gene mutated |
Upstream/ Exon/ Intron/ Downstream | Chromosome location of the gene |
Annotation |
Confirmation of the location |
|---|---|---|---|---|---|---|---|
| T323 | y20 (Ames 2) mdh1-n (Ames 2) | Gm12: 27,790,785–27,790,786 | Glyma.12G159300 | 1st Intron | Gm12: 27,787,400–27,791,365 | Lactate/malate dehydrogenase | Located 1.9 Mb from Satt253 |
| T325 |
y20 (Ames 4) mdh 1-n (Ames 4) |
Gm12: 27,790,785–27,790,786 | Glyma.12G159300 | 1st Intron | Gm12: 27,787,400–27,791,365 | Lactate/malate dehydrogenase | Located 1.9 Mb from Satt253 |
| T346 | y20 (Ames 17) mdh1-n (Ames 19) | Gm12: 27,790,785–27,790,786 | Glyma.12G159300 | 1st Intron | Gm12: 27,787,400–27,791,365 | Lactate/malate dehydrogenase | Located 1.9 Mb from Satt253 |
| T359 | ms9 | Gm03: 36,726,043–36,726,044 | Glyma.03G152300 | 1st intron | Gm03: 367,24,492–36,727,791 | No Functional annotation | Located 1.4 Mb from Satt237 |
| T364 | fsp2 | Gm06: 14,708,172–14,708,173 | Glyma.06G174200 | 7th intron | Gm06: 14,707,013–14,712,446 | Haloacid dehalogenase-like hydrolase | Located 3.4 Mb from Satt170 |
| T365 | fsp3 | Gm08: 47,100,615–47,100,616 | Glyma.08G359000 | 2nd exon | Gm08: 47,099,991–47,102,594 | Embryo-specific protein 3, (ATS3) | Located 115 kb from Satt429 |
| T367 | fsp5 | Gm18: 39,611,234–39,611,235 | Glyma.18G169500 | 272 bp upstream | Gm18: 39,611,506–39,611,853 | Embryo-specific protein 3, (ATS3) | Located between satt324 and satt138 |