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. 2017 Aug 10;12(8):e0182704. doi: 10.1371/journal.pone.0182704

Fig 4. Accumulation of fat infiltrates in LGMD strains.

Fig 4

(a) Immunofluorescent images of skeletal muscles stained with Nile-red (infiltrated lipids, red) Scale bar: 100 μm (b) Quantification of the immunofluorescent areas. A significant increase in Nile red -positive areas (n = 5 mice per group) was found in SGCD-null diaphragm and SGCA-null gastrocnemius muscles when compared to wild type muscles. (c) Adipogenic gene expression measured by qPCR, normalized to Gapdh (n = 5 mice per group). Upregulation of adipogenic gene expression (Adipoq, Ppar-gamma, Atgl) was found in diaphragm muscle of both LGMD strains and Hsl was significantly upregulated in SGCA-null diaphragm muscle compared to wild type. Q, quadriceps; GC, gastrocnemius; TA, tibialis anterior; Dia, diaphragm. * Indicates significant difference from WT controls. Error bars represent ± SD.