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. 2017 Aug 10;12(8):e0183101. doi: 10.1371/journal.pone.0183101

Fig 2. Chlamydial antigens measured on captured EBs.

Fig 2

A total of 4.5 μg/ml of non-pooled or pooled EBs (red symbols) were fixed to poly-L-lysine-coated plates then incubated with duplicate serial dilutions of antibodies against chlamydial outer membrane proteins: (A) Non-pooled EBs of serovar D with mouse anti-CT LPS, (B) Non-pooled EBs of serovar E with mouse anti-CT LPS (C) Pooled EBs of serovars D and E with mouse anti-LPS (D) Pooled EBs of serovars D and E with mouse anti-MOMP D (E) Pooled EBs of serovars D and E with mouse anti-MOMP E and (F) Pooled EBs of serovars D and E with mouse anti-OmcA. Antibodies against cytoplasmic antigens of pooled EBs of serovars D and E: (G) Rabbit anti-Euo, (H) Mouse anti-Hsp60, and (I) Rabbit anti-Scc4. 4.5 μg/ml of lysed EBs of pooled serovars D and E were evaluated with (J) Rabbit anti-Euo (K) Mouse anti-Hsp60 and (L) Rabbit anti-Scc4. Blue symbols represent results obtained with irrelevant isotype-matched antibodies. Mean absorbance values ± SD from one representative experiment of three are shown.