| human α7 nAChR subunit |
| Q117T-F |
5’-gcattgcacgtacctgcct-3’ |
| Q117T-R |
5’-aggcaggtacgtgcaatgc-3’ |
| Y118W-F |
5’-cattgccagtggctgcctcca-3’ |
| Y118W-R |
5’-tggaggcagccactggcaatg-3’ |
| S184N-F |
5’-gcaagaggattgaaaggttcta-3’ |
| S184N-R |
5’-tagaacctttcattcctcttgc-3’ |
| E185V-F |
5’-gcaagaggagtgtaaggttcta-3’ |
| E185V-R |
5’-tagaaccttacactcctcttgc-3’ |
| F187S-F |
5’-agtgaaaggtcttatgagtgc-3’ |
| F187S-R |
5’-gcactcataagacctttcact-3’ |
| E189G-F |
5’-aggttctatggatgctgcaa-3’ |
| E189G-R |
5’-ttgcagcattcatagaacct-3’ |
| rat α7 nAChR subunit |
| L119D-F |
5’-cagtacgatcctccaggcatattc-3’ |
| L119D-R |
5’-ggaggatcgtactggcaatg-3’ |
| chimeric α7/GlyR subunit |
| E189A-F |
5’-cttttatgcgtgctgtaaagaac-3’ |
| E189A-R |
5’-cttgactctcgaaaatacgcacgac-3’ |
| mouse α nAChR subunit |
| G153S-F |
5’-ctatgacagctctgtggtggc-3’ |
| G153S-R |
5’-cacagagctgtcataggtccag-3’ |
| Y190F-F |
5’-gtgttcttctcctgctgccc-3’ |
| Y190F-R |
5’-gcaggagaagaacacccagtg-3’ |
