Table 4. Agonist affinity to WT and mutant muscle nAChRs measured by calcium imaging of cell populations with calcium ion indicators Case12 and Fluo-4.

Receptor	Calcium imaging
	Acetylcholine,
	Mean EC50 (95% confidence interval)
	Case12	Fluo-4
muscle nAChR	4.39 (3.18–6.06) μM	8.47 (6.24–11.51) μM
muscle nAChR [G153S]	0.27 (0.19–0.39) μM	0.23 (0.08–0.62) μM
muscle nAChR [Y190F]	5.58 (3.8–8.2) mM