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. 2017 Aug 10;12(8):e0182679. doi: 10.1371/journal.pone.0182679

Fig 1. Glutamine is essential for HSCs proliferation.

Fig 1

A&B: relative proliferation of LX2 cells (A,B) or primary HSCs (D) by BrdU staining or cell counting (C). Cells were plated in complete medium or Gln deficient medium with or without α-KG, NEAA, or the mixture of the two. BrdU-positive cells were quantified per optical section. Relative BrdU-positive cells for each group were analyzed. Error bars represent s.d. of triplicate samples from a representative experiment. *P < 0.05.