Fig 5. Competition ELISA to estimate relative affinity of vaccine-induced antibodies.

Antigens in the form of peptides or recombinant monomer/oligomer protein preparations as described in the methods were coated on to microtitre plates and ELISA reading measured with fixed concentrations of serum IgG antibodies (at OD50 dilution) purified from pooled sera of vaccinated mice (n = 4), preincubated with serial dilutions of (A) free a-syn peptides (PD1, left; PD3, right); (B) free a-syn protein, either monomeric (left panels) or oligomeric (right panels) species, PD1 (top) and PD3 (bottom); and (C) liberated a-syn from haemolysed RBCs (orange line) or PBS negative control (blue line) for PD1 (top) and PD3 (bottom).