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. 2017 Aug 10;12(8):e0181844. doi: 10.1371/journal.pone.0181844

Fig 8. Effects of long-term immunisation with Qb-PD vaccines on a-syn aggregates.

Fig 8

Male and female SNCA-OVX mice received 20 μg of Qb, Qb-PD1, Qb-PD3 or PBS every two weeks for a month, followed by monthly injections for 12 months (total duration of immunisation: for 13 months). (A-D) Immunofluorescence analysis was performed to detect a-syn aggregates with PLA in the substantia nigra, striatum, hippocampus and cerebellum. PLA puncta were quantified using ImageJ. (E) PLA puncta in 26–36 TH positive neurons were quantified and expressed as average mean of puncta per positive cell ± SEM (n = 3–4 mice per group), ANOVA followed by Dunn’s post hoc test. Scale bar 50 μm.