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. 2017 Aug 10;12(8):e0182821. doi: 10.1371/journal.pone.0182821

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© 2017 Mohammad et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (a) Diagram of key reactions in peptidoglycan synthesis for bacterial cell wall. Abbreviations: MurA, UDP-N-acetylglucosamine enolpyruvyl transferase; MurB, UDP-N-acetylenolpyruvylglucosamine reductase; MurC, UDP-N-acetylmuramoyl-L-alanine synthetase; MurD, UDP-N-acetylmuramyl-l-alanine:d-glutamate ligase; MurE, UDP-N-acetylmuramoyl-l-alanyl-d-glutamate:meso-2,6-diaminopimelate ligase, MurF, UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase, MurG, UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; IPP, isopentenyl diphosphate, DMAPP, dimethylallyl diphosphate; FPP, isoprenoid farnesyl diphosphate; FPPS, farnesyl diphosphate synthase; UPP, C₅₅ isoprenoid undecaprenyl diphosphate; UPPS, undecaprenyl diphosphate synthase; UPPP, undecaprenyl diphosphate phosphatase; UP, undecaprenyl monophosphate; MraY, phospho-MurNAc-pentapeptide translocase; GlcNAc, N-acetylglucosamine; MurNAc, N-acetylmuramic acid; PBPs, penicillin-binding proteins. (b) Detection of final soluble cell wall precursor (UDP-N-acetylmuramyl pentapeptide) inside bacterial cytoplasm. HPLC chromatogram of S. aureus NRS107 (RN4220) treated with 10 × MIC of compound 1 or vancomycin for 30 minutes. After centrifugation, the bacterial pellet was boiled for 30 minutes to release contents present in the bacterial cytoplasm. The lysate was analyzed using HPLC/MS, using a phenyl column, to determine the accumulation of the final soluble precursor in cell wall synthesis, UDP-N-acetylmuramyl pentapeptide (designated by the black arrow).