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. 2017 Aug 10;12(8):e0182821. doi: 10.1371/journal.pone.0182821

Fig 7. Examination of activity of diphenylurea compounds against mature staphylococcal biofilm.

Fig 7

Detection of ability of diphenylurea compounds and vancomycin to (a) penetrate mature biofilm to inhibit/kill S. epidermidis NRS101 CFU present in biofilm and (b) disrupt integrity of mature biofilm of S. epidermidis NRS101 evaluated via crystal violet reporter assay. Detection of ability of diphenylurea compounds and vancomycin to (c) penetrate mature biofilm to kill S. aureus ATCC 6538 CFU present in biofilm and (d) disrupt mature biofilm of S. aureus ATCC 6538. Untreated wells served as a negative control. The values represent an average of a minimum of three samples analyzed for each compound/vancomycin. Error bars represent standard deviation values. Asterisk (*) denotes statistical significance between wells treated with compounds 1, 2, or vancomycin compared to untreated wells as determined by a two-way ANOVA with Dunnet’s multiple comparisons test (n = 3, P < 0.05).