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. 2017 Mar 3;15(9):1120–1129. doi: 10.1111/pbi.12702

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© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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UPLC chromatograms presenting the peaks of the main glycans released from a DNaseI protein (top to bottom): Glycan pool derived from DNaseI produced by control cells (WT); glycan pool from a XylT knocked‐out line (ΔXT); glycan pool released from DNaseI produced from a FucT knocked‐out line (ΔFT); and a glycan pool released from DNaseI from a XylT/FucT knocked‐out cell line (ΔXFT). Glycans are annotated with cartoons and acronyms above main peaks. Symbols are as follows: ‐Mannose; ‐Xylose; ‐N‐acetylglucosamine; ‐Fucose. Glycan acronyms are based on the nomenclature indicated at the following website http://www.proglycan.com/sites/default/public/pdf/nomen_2007.pdf.

Inline graphic — UPLC chromatograms presenting the peaks of the main glycans released from a DNaseI protein (top to bottom): Glycan pool derived from DNaseI produced by control cells (WT); glycan pool from a XylT knocked‐out line (ΔXT); glycan pool released from DNaseI produced from a FucT knocked‐out line (ΔFT); and a glycan pool released from DNaseI from a XylT/FucT knocked‐out cell line (ΔXFT). Glycans are annotated with cartoons and acronyms above main peaks. Symbols are as follows: ‐Mannose; ‐Xylose; ‐N‐acetylglucosamine; ‐Fucose. Glycan acronyms are based on the nomenclature indicated at the following website http://www.proglycan.com/sites/default/public/pdf/nomen_2007.pdf.