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. 2017 Mar 3;15(9):1120–1129. doi: 10.1111/pbi.12702

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© 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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UPLC chromatograms presenting the peaks of the main glycans released from a DNaseI protein (top to bottom): Glycan pool derived from DNaseI produced by control cells (WT); glycan pool from a XylT knocked‐out line (ΔXT); glycan pool released from DNaseI produced from a FucT knocked‐out line (ΔFT); and a glycan pool released from DNaseI from a XylT/FucT knocked‐out cell line (ΔXFT). Glycans are annotated with cartoons and acronyms above main peaks. Symbols are as follows: ‐Mannose; ‐Xylose; ‐N‐acetylglucosamine; ‐Fucose. Glycan acronyms are based on the nomenclature indicated at the following website http://www.proglycan.com/sites/default/public/pdf/nomen_2007.pdf.

Inline graphic — UPLC chromatograms presenting the peaks of the main glycans released from a DNaseI protein (top to bottom): Glycan pool derived from DNaseI produced by control cells (WT); glycan pool from a XylT knocked‐out line (ΔXT); glycan pool released from DNaseI produced from a FucT knocked‐out line (ΔFT); and a glycan pool released from DNaseI from a XylT/FucT knocked‐out cell line (ΔXFT). Glycans are annotated with cartoons and acronyms above main peaks. Symbols are as follows: ‐Mannose; ‐Xylose; ‐N‐acetylglucosamine; ‐Fucose. Glycan acronyms are based on the nomenclature indicated at the following website http://www.proglycan.com/sites/default/public/pdf/nomen_2007.pdf.