Figure 3.

Introduction of (SP)_n glycomodules enhances the yields of secreted Venus in C. reinhardtii. (a) Secretion yields of selected transgenic lines determined by dot blotting. Concentrations of Venus in the culture media from five lines for each construct were quantified using purified E. coli‐derived Venus as a standard. Supernatants from SSVenus‐(SP)₁₀ and SSVenus‐(SP)₂₀ selected lines were collected after 7 days of cultivation and used directly for dot‐blot analysis. Means of three technical replicates and standard errors are shown. Statistical analysis was performed using a one‐way ANOVA (P < 0.05). The asterisk represents a significant difference from SS01. (b). Fluorescence emission from cells grown on agar plates detected with a stereo fluorescence microscope. Fluorescence was detected under the same settings for all colonies. i, untransformed UVM4; iii, Venus; iii, SSVenus; iv, SSVenus‐(SP)₁₀; v, SSVenus‐(SP)₂₀. Scale bars indicate 1 mm.