Table 1.
Oligonucleotide primers used in this study for cloning, RNAi and RT-PCR.
| GenBank accession No. | Oligonucleotide sequence (5′-3′ for forward and reverse primers) |
|---|---|
| CLONING | |
| ISCW024685 | CACCATGAAAAGCAGCGCACTGCTG |
| GCGTTTACCACGAACGCACC | |
| ISCW024295 | CACCATGCCGAAACAAGGCGAAAC |
| TCCAGAGTCACCACACAAAACG | |
| ISCW022212 | CACCATGTGGGGTCAGATTGCGCT |
| ACAGATGAATTTTTTCAGGC | |
| ISCW020900 | CACCATGAACAAAGCGATCTTCAT |
| CACTTCACCGAAAAAGCCGC | |
| ISCW000326 | CACCATGCCGGCGTCAATGAAAAG |
| CAGAGAACCCAGATTCGGAA | |
| ISCW008146 | CACCATGGATTTTGATGACCTGTT |
| GAAGCTCAGGGTGTTCTGTT | |
| ISCW008641 | CACCATGCAACGTGACATTTTTAG |
| CCAACAGCCCGGCTGCGATT | |
| ISCW024499 | CACCATGTGCCTGGTGTTTGCAAC |
| GCGCAGAAAGGAACTCGTAC | |
| ISCW005600 | CACCATGATTCGTCAGGTTCGCGA |
| CGAACCTGAGATCGATGAGG | |
| ISCW013709 | CACCATGTTTCGTACCAGCTCTGG |
| CACAATATAATCCGGTGCAC | |
| ISCW017117 | CACCATGCTGAGTGTGCTGCTGGG |
| CGTGGTGGCGTCCGGCGGCG | |
| ISCW013574 | CACCATGTATCAGCTGCGCGATTT |
| GCAACGGGATTTGCGAACAC | |
| ISCW018900 | CACCATGGGCCCGTTTATTGGTCT |
| GCCGATAATGCGACCGATAA | |
| ISCW023907 | CACCATGCCGGTCAATCGCCTGAT |
| AACTTTACGAAAGAAAAACA | |
| ISCW024682 | CACCATGATTCATGAACCGGTGAT |
| ATACGGACAGTACAGTTTGCA | |
| ISCW015453 | CACCATGATGAAAAGCCCGCTGTTTAT |
| ACCGAAAAAGCCGTGGCCGA | |
| ISCW021670 | CACCATGTGGGAACTGCATGCCGA |
| CTCCTGGGTAATATTACGCGT | |
| ISCW017271 | CACCATGTGCAGCGATTCTAAACC |
| CGGCAGATAGGAACCGTGCG | |
| AAY66632 | CACCATGGGCCTGACCGGTACCAC |
| GTTGTCTTTGGTTTTCTTGG | |
| RNAi AND RT-PCR | |
| ISCW005600 | TCCCCTTCTCAAAGGAGGAT* |
| ATCCACAGGCGGATATGAAG* | |
| AAY66632 | ACCCGTTCATGGGACAAATA* |
| TTCTTGGGCTTCTCAGTTGG* | |
| DQ066214 (rpS4) | GGTGAAGAAGATTGTCAAGCAGAG |
| TGAAGCCAGCAGGGTAGTTTG | |
*
The same oligonucleotide primers were used to determine gene expression levels by RT-PCR and for the generation of dsRNA for RNAi. To produce dsRNA, the T7 promoter sequence 5′-GAATTAATACGACTCACTATAGGGAGA-3′was added to the 5′-end of each primer.