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. 2017 Aug 10;7:7837. doi: 10.1038/s41598-017-08319-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of TonEBP haploinsufficiency on hepatic steatosis in HFD/STZ-treated mice. (a) Representative micrographs of H&E and Nile red staining; scale bar = 50 μm. (b) Percentages of Nile red-positive areas. (c) Concentrations of hepatic TG (n = 7–8 mice per group). Western blotting and quantification of nuclear LXRβ (d), SREBP-1 (e), and PPARα (f) in the livers. The mean values were obtained from three separate experiments (n = 6 mice per group). Cropped blots are displayed here and full-length blots are included in the Supplementary Information. Data are presented as the mean ± SEM. *P < 0.05 vs. control (CTL) normal diet-fed mice; ^† P < 0.05 vs. DM WT mice.