Table 4.
RNA total amounts obtained from plasma samples according to the Qubit assay.
| ID plasma pool | Starting plasma volume (µL) | Sample volume after RNA isolation (µL) | RNA quantification by Qubit (ng/µL) | Total RNA content (ng) |
|---|---|---|---|---|
| 1Q | 400 | 28 | 1.60 | 44.74 |
| 2Q | 400 | 28 | 1.46 | 40.88 |
| 3Q | 1200 | 72 | 0.90 | 64.80 |
| 4Q | 1600 | 100 | 0.98 | 98.00 |
| 5Q | 1200 | 72 | 1.17 | 84.24 |
| 6Q* | 2000 | 132 | 0.92 | 121.44 |
| 7Q* | 2000 | 132 | 0.81 | 107.45 |
| 8Q | 1600 | 104 | 0.76 | 79.25 |
| 9Q | 1600 | 104 | 0.81 | 84.66 |
| 10Q | 1200 | 76 | 0.72 | 55.02 |
| 11Q | 1200 | 76 | 0.76 | 57.91 |
| 12Q* | 2000 | 117 | 1.65 | 192.58 |
| 13Q* | 2000 | 117 | 0.82 | 95.94 |
| 14Q* | 2000 | 117 | 1.31 | 152.88 |
| 15Q* | 2000 | 117 | 1.04 | 121.45 |
| 16Q* | 2000 | 117 | 1.12 | 131.35 |
| 17Q* | 2000 | 117 | 1.14 | 133.61 |
| 18Q* | 2000 | 117 | 1.04 | 121.99 |
| 19Q* | 2000 | 117 | 1.35 | 158.42 |
| 20Q* | 2000 | 117 | 1.10 | 129.25 |
| Mean (SD)# | 1.12 (0.25) | 133.31 (26.50) |
#Mean and SD values of samples with starting plasma volume of 2 mL (denoted with*).