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. 2017 Aug 10;7:7817. doi: 10.1038/s41598-017-07532-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MKN-28 mRNAs levels of FPR1, FPRL1, FPRL2 and CTSG. Results are representative of three independent experiments. Each value is the mean ± SD of three replicas. Expression values were normalized against the human glyceraldehydes-3-phosphate dehydrogenase (GAPDH) gene. Stability assay, carried out using the BestKeeper tool, indicated that GAPDH was more stable then ACT-β at 30 (1.03 vs 2.89) and 60 min (1.89 vs 3.29). Statistical analysis was carried out with the GraphPad Prism version 5.03 (GraphPad, La Jolla, CA, USA). Differences in expression levels between 30 min and 1 h are all significantly different (P < 0.001); ^aHp(2-20) concentration was 2 × 10⁻⁵ M; ^bHpgb = H. pylori growth broth (140 µl/well).