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. 2017 Aug 9;8:1515. doi: 10.3389/fmicb.2017.01515

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Copyright © 2017 Rosani and Venier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Coverage graph of Malacoherpesviridae reads (A). Coverage graph of the whole OsHV-1 genome, as obtained by mapping 4.9 M viral reads with length and similarity mapping parameters set to 0.9. Yellow arrowheads indicate annotated ORFs along the virus genome. Maximum coverage was set to 10,000x. (B) Details of the ORF104 coverage graph, with the alignment including the 9-nt deletion causing coverage drop-off. (C) Details of ORF107 coverage graph, with two nested ORFs (arrows) that could explain the local coverage bias. One ORF encoded three transmembrane regions (highlighted in blue).