Table 2.
Primers used in this study.
| Name | Oligonucleotide sequence (5′–3′)a | Product |
|---|---|---|
| CONSTRUCTION OF RECOMBINANT PLASMID | ||
| hsdS′-UF | GCCGGATCCCCAATAAAGAGACCGTGT | Upstream of hsdS′ |
| hsdS′-UR | CTATCGAGAAATGAAAGATTCAATTTCTTGGACTGT | Upstream of hsdS′ |
| hsdS′-DF | ACAGTCCAAGAAATTGAATCTTTCATTTCTCGATAG | Downstream of hsdS′ |
| hsdS′-DR | GCCGTCGACGGTTTTGGAGATACACTT | Downstream of hsdS′ |
| hsdS′-F | GGAGTGGGTGAGGTTGGGT | Part of hsdS′ |
| hsdS′-R | TCAACTTTGTTTTTGGAAT | Part of hsdS′ |
| lysM-UF | GCGGTCGACGAGGAAAATCAGGTCGTCTTG | Upstream of hsdS |
| lysM-UR | GCTGAAACGGTTATTTTTGTAAGTAATCTTATTCCTTCATTATTTTTG | Upstream of hsdS |
| lysM-DF | CAAAAATAATGAAGGAATAAGATTACTTACAAAAATAACCGTTTCAGC | Downstream of hsdS |
| lysM-DR | CGGGAATTCTCAATGCCTACCAGATGCTC | Downstream of hsdS |
| lysM-F | CTTCATTGGCTCTTTCCCTC | Part of hsdS |
| lysM-R | ACGGTCTGGCATCAGGTTC | Part of hsdS |
| lysM′-UF | GCGGTCGACAATGCCAAAATGGGCTTAGT | Upstream of hsdM |
| lysM′-UR | ATGACAGAAAGGAATGTGACTATTGATAAATTAGAAGAGGTCTAGCACG | Upstream of hsdM |
| lysM′-DF | CGTGCTAGACCTCTTCTAATTTATCAATAGTCACATTCCTTTCTGTCAT | Downstream of hsdM |
| lysM′-DR | CGGGAATTCGAAGAGGCTTTGTATTTGAACG | Downstream of hsdM |
| lysM′-F | GCATCTGCTACACGCTCTT | Part of hsdM |
| lysM′-R | CAACTATTGCTGGTTTGGT | Part of hsdM |
| hsdS-CF | GCCGGATCCGACGAAAAAAACAGAAGA | hsdS and its promoter |
| hsdS-CR | GCCGAATTCTCAGTAATAAAGTTGGGC | hsdS and its promoter |
| hsdS′-CF | GCCGGATCCGTCTCCTTTGAAGTAAAACGAA | hsdS′ and its promoter |
| hsdS′-CR | GCCGAATTCTTCAGGATTATTGATACCTATTTCT | hsdS′ and its promoter |
| qPCR | ||
| ZY05719_RS01205-F | TGTCTACGCTCTGTCCTTTG | Part of ZY05719_RS01205 |
| ZY05719_RS01205-R | AAATCCCTACCTGCTCGTC | Part of ZY05719_RS01205 |
| lysM-F | CTTGCTGGCGAAAATAAATC | Part of lysM |
| lysM-R | CAGTTGCCTGAACGGTCTC | Part of lysM |
| ZY05719_RS01215-F | TACCGATCTCCTCACCCAT | Part of ZY05719_RS01215 |
| ZY05719_RS01215-R | AACAACCACGACCAACCTC | Part of ZY05719_RS01215 |
| ZY05719_RS10230-F | TGTCAGATTCCGCTGTCCT | Part of ZY05719_RS10230 |
| ZY05719_RS10230-R | GCTTTTCAATAGCAACTCCT | Part of ZY05719_RS10230 |
| lysM′-F | ATCTGCTACACGCTCTTGG | Part of lysM′ |
| lysM′-R | CGTAACGGTATCTACATTGGT | Part of lysM′ |
| ZY05719_RS10240-F | CCTTCTGAATAAGATTTCCCTC | Part of ZY05719_RS10240 |
| ZY05719_RS10240-R | CTTGCGTTTTGTACCGACC | Part of ZY05719_RS10240 |
| 16S RNA-F | GTTGCGAACGGGTGAGTAA | Part of 16S RNA |
| 16S RNA-R | TCTCAGGTCGGCTATGTATCG | Part of 16S RNA |
a
Underlined portions of the primers correspond to restriction enzyme recognition sites. GTCGAC, Sal I; GGATCC, BamH I; and GAATTC, EcoR I.