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. 2017 Aug 9;8:528. doi: 10.3389/fphar.2017.00528

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Copyright © 2017 Liu, Fang, Yuan, Li, Yang, Jiang, Ouyang and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of naked pDNA, PEI (25k Da)/pDNA, PDAPEI/pDNA polyplexes at 10, 20, 30, 40, and 50 w/w ratios. Transfection efficiency in HUVECs was quantified by the flow cytometer in the form of GFP positive cells.