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. 2017 Aug 9;8:1514. doi: 10.3389/fmicb.2017.01514

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Copyright © 2017 Xin, Cao, Liu, Ren, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Test for mechanical transmission of watermelon crinkle leaf-associated virus 1 (WCLaV-1) to watermelon. (A,B) RT-PCR and agarose gel electrophoresis to detect infected watermelon leaves. (A) At 17 days post-inoculation (dpi), only sample No. 4 was infected; (B) detection of systematic infection in four leaves of sample No. 4 using two primer pairs in RT-PCR. Symptom development over time (C–E). (C) Crinkling at 17 dpi; (D) mosaic and stunt at 42 dpi compared with the non-inoculated plant and close ups of symptoms; (E) curling and mosaic at 67 dpi. M, DNA Marker DL2000 (Takara); PC, positive control; NC, uninfected watermelon control; WC, water control.