Table 5.

Primer sequences of the virulence related genes of Foc and EF1α (used as a reference gene) used for qRT-PCR.

Target gene	Forward primer sequence (5′ → 3′)	Reverse primer sequence (5′ → 3′)	Amplicon size
Class V Chitin synthase	GGCCTACATCAACTCTGCAAC	GGGCATTATAACGACCGTCTCAA	96 bp
Ubiquitin fusion protein	CAACCCCAATTCGCACCATC	CCGTGAGGGTCTTGACGAAA	96 bp
Chitin synthase 4	CGGATTATGGGGGAAACCATGT	TTGGCCTCAAGAATGTTACCCCTT	99 bp
Worronin body major protein	ACCCGCTCCCCAATTCTATT	GGTTGTACTGAGGGCGAGAT	86 bp
ABC transporter CDR4	GATTCACCCCTTAACCCGCA	CTGTCGAAACCCAGAGCCAT	99 bp
ATP synthase	CAATGTTTGCATGCCCGTCT	CGTTGACACCAGCGAAGATG	98 bp
Β-glucosidase	CTGTTCACCGAGTGCATCCT	AAATCACCGTTGCCATTGCC	91 bp
60S ribosomal protein	GTGCCCTCAAGTACGTCGAA	ATTGACGGAGTTCCCAGCAG	93 bp
EF1α	AGCTCGGTAAGGGTTCCTTC	TCCAGAGAGCAATATCGATGG	93 bp