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. 2017 Aug 10;7:7846. doi: 10.1038/s41598-017-08255-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AFM-images of typical nanosurfaces of II order of PRBC membranes and corresponding nanosurface profiles. (a) For discocyte on the 5^th day of PRBC storage. (b) Single topological defects (highlighted by yellow circles) on the surface of membranes on the 16^th day of PRBC storage. (c) Domains with a grain-like structure.(d) Spheroehinocyte on the 33^th day of storage and its nanosurface. (e) Swelled cells e on the 40^th day of storage and its nanosurface.