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. 2017 Aug 10;7:7758. doi: 10.1038/s41598-017-08227-z

Figure 3.

Figure 3

TPPU attenuates bilateral carotid artery stenosis (BCAS) induced white matter damage and promotes functional recovery. (A) Representative immunofluorescent staining of myelin associated glycoprotein (MAG, red), myelin basic protein (MBP, green), and non-phosphorylated neurofilaments (SMI32, red) in the corpus callosum (CC) of mice in sham, vehicle and TPPU treatment mice at 4 weeks after operation, n = 5/group. Scale bar represents 100 μm. (B–E) Representative and statistical analysis of western blots of MAG, MBP, SMI32, and β-actin expression in the CC of sham, vehicle and TPPU treatment groups 4 weeks after operation, n = 5/group. (F) Representative LFB staining in the CC of sham, vehicle and TPPU treatment groups at 4 weeks after operation, n = 5/group. Scale bar represents 50 μm. (G) Representative electron micrographs of white matter in the CC from sham, vehicle and TPPU treatment groups at 4 weeks after operation, n = 4/group. Scale bar represents 1 μm. (H–J) Statistical analysis of axon diameter/fiber diameter (G-ratio) and fiber diameter in the sham, vehicle and TPPU treatment groups at 4 weeks after operation. (K–M) The eight-arm radial maze test (Revisiting errors, Different arm choices in the first 8 entries, and Reference errors) was performed to measure cognitive deficits at 4 weeks in sham, vehicle and TPPU treatment groups, n = 10/group. Values are expressed as mean ± SEM *P < 0.05, **P < 0.01, vs sham group. # P < 0.05, ## P < 0.01, vs vehicle group.