Figure 5.

TPPU reduces the microglia activation and inflammatory response after bilateral carotid artery stenosis (BCAS). (A) Representative immunofluorescent staining of Iba1 (red) in the corpus callosum (CC) of mice in sham, vehicle and TPPU treatment groups from days 3 to weeks 8 after operation. Scale bar represents 50 μm. (B) Representative immunofluorescent staining of Iba1 (red) and CD68 (green) in the corpus callosum (CC) of mice in sham, vehicle and TPPU treatment groups at 4 weeks after operation. Scale bar represents 50 μm. (C) Statistical analysis of Iba1 labeled microglia in the CC areas at weeks 2 and weeks 4 were expressed as cells/mm², n = 5/group. (D) Statistical analysis of Iba1/CD68 labeled microglia in the CC areas at weeks 4 were expressed as cells/mm², n = 5/group. (E) Representative western blots of TNF-α, IL-1β, and β-actin expression in the CC of sham, vehicle and TPPU treatment groups at 4 weeks after operation. (F–G) Statistical analysis of western blots signals of TNF-α and IL-1β in the CC of sham, vehicle and TPPU treatment groups, n = 5/group. Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01, vs sham group. ^# P < 0.05, ^## P < 0.01, vs vehicle group.