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. 2017 Aug 10;7:7833. doi: 10.1038/s41598-017-07947-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibiting either the AKT or MTOR pathways alters the mechanical properties of LNCaP cells. Variation in (A) shear modulus or (B) viscosity of shRb and shSCX LNCaP cells after exposure to 72 h of hypoxia or normoxia and treated with or without 2 μM A6730. (C) Immunoblots of LNCaP shRb and shSCX whole cell lysates after exposure to 72 h of hypoxia or normoxia and treated with or without 2 μM A6730. Blots were probed with antibodies against AKT, phospho-AKT (Thr308), phospho-AKT (Ser473) and α-tubulin. Samples were derived from the same experiment and blots were processed in parallel. Complete blot images are shown in Supplementary Information File 1, Figure S1. Variation in (D) shear modulus or (E) viscosity of shRb and shSCX LNCaP cells after exposure to 72 h of hypoxia or normoxia and treated with or without 10 nM INK128. For A, B, D and E, experiments were conducted in triplicate (n = 3) and ten cells were examined in each experiment. Error bars represent ± S.D. *p < 0.05.