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. 2017 Aug 10;7:7833. doi: 10.1038/s41598-017-07947-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence microscopy of actin in LNCaP cells. shRb and shSCX LNCaP cells were exposed to 72 h of hypoxia or normoxia and treated with or without 10 µM U0126 or 2 µM A6730. Fixed cells were treated with DAPI and TRITC-labelled rhodamine phalloidin and were imaged with a Zeiss LSM-780 confocal microscope. Phalloidin only images are represented in the left panels, merged images are in the middle panels and enlarged merged images are represented in the right panels. Filopodia-like processes are highlighted with large white arrow heads and cell:cell actin borders are shown with white arrows. Each experiment was repeated four times.