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. 2017 Aug 10;7:7833. doi: 10.1038/s41598-017-07947-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exposure to hypoxia increased the total number of migrating shRb LNCaP cells. (A) Immunoblot of LNCaP shRb and shSCX whole cell lysates after exposure to 72 h of hypoxia or normoxia and probing with primary antibodies to pan-actin or α-tubulin. Samples were derived from the same experiment and blots were processed in parallel. Complete blot images are shown in Supplementary Information File 1, Figure S1. (B) Total number of migrating cells for shRb and shSCX LNCaP cells after exposure to hypoxia or normoxia. (C) Total number of migrating cells for shRb LNCaP cells conditioned with hypoxia and treated with DMSO, 10 μM U0126, 2 μM A6730 or 10 nM INK128. Experiments were performed in triplicate. Error bars represent ± S.D. *p < 0.05.