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. 2017 Aug 10;7:7743. doi: 10.1038/s41598-017-08089-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Levels of normally segmented granulocytes in each layer. (A) Representative photography of cytospins (x400, stained Protocol Hema 3) of the peripheral blood mononuclear cell layers (scale bar = 28 µm). Mature granulocytes (empty arrow) have more than 2 nuclear lobes (classically between 3 and 4) connected by filaments, whereas immature granulocytes (full arrow) have a curved nucleus with 2 or fewer nuclear lobes. LDNs were quantitated morphologically by light microscope. (B) Percentages of normally segmented LDNs in peripheral blood mononuclear cells of controls and horses with severe asthma. Each symbol denotes a single animal (mean ± SEM). *p < 0.05 compared with control.