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. 2017 Aug 10;7:7743. doi: 10.1038/s41598-017-08089-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neutrophil extracellular traps (NETs) production in both layers of cells. (A) Representative photography of using a MRC1024 confocal laser-scanning microscope at ×100 magnification (BioRad, Hercules, CA) equipped with a Nikon Eclipse TE300 camera (Nikon, Tokyo, Japan) of the low-density layer (scale bar = 100 µm). White arrows indicate NETs’ structures. (B) Log transform of the mean area of NETs per neutrophil in each layer (non-stimulated NS). Each symbol denotes a single animal. Mean ± SEM for each studied population is shown. *p ≤ 0.05.