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. 2017 Aug 10;7:7743. doi: 10.1038/s41598-017-08089-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fold changes of mean area of NETs per neutrophil in each layer of cells. (A) Results represent fold induction of the effects of PMA stimulation on the mean area of NETs per neutrophil on each layer of cells in each group of horses. Stimulation with PMA significantly increased the production of NETs in both groups of horses (p = 0.0008). Mean ± SEM for each studied population is shown. A two-way repeated measures ANOVA without Sidak’s multiple comparison post-tests has been realized in this case. (B) Results represent fold induction of the mean area of NETs per neutrophil by LDNs compared to NDNs in each group of horses, before and after stimulation with PMA. LDNs produced significantly more NETs than NDNs (p < 0.001). Mean ± SEM for each studied population is shown. *p ≤ 0.05 and **p ≤ 0.01.