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. 2017 Aug 10;7:7850. doi: 10.1038/s41598-017-08092-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Cell viability (yellow), oxidative stress (green) and mitochondrial health (red) of hMSCs labelled with IONPs at various concentrations and determined by high-content imaging reveals significant induction of reactive oxygen species (ROS; green colour) at 10 and 50 µg Fe/ml. Scale bar 100 µm. (b) Relative viability and (c) production of reactive oxygen species determined by high-content imaging. Data are represented relative to untreated control cells as mean ± SD for minimum 500 cells per condition, scale bar 100 µm. The degree of significance is indicated when appropriate *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA, Dunnett post-hoc test).