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. 2017 Aug 10;7:7850. doi: 10.1038/s41598-017-08092-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the signal intensity of the specific region of interest defined as the liver, as evaluated from the MR images in mice administered PBS (control) and mice administered 60 µg Fe of IONP-DHCA after 1 d, 1 wk and 2 wk post IONP exposure. The signal was normalized against muscle tissue around the vertebra column. The analysis was conducted in replicates of three mice per condition over 2 wk. The degree of significance is measured using GraphPad Prism software and is indicated when appropriate *P < 0.05, **P < 0.01 and ***P < 0.001 (one-way ANOVA, Dunnett post-hoc test).