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. 2017 Aug 10;7:7728. doi: 10.1038/s41598-017-07938-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Depletion of CD71⁺ cells enhances IL-17, IFN-γ secreting and antibody producing adaptive immune cells following immunization. (A) The cartoon shows treatment and vaccination time points. (B) Representative plots showing IL-17 and IFN-γ producing CD4⁺ T cells among splenocytes following stimulation with HKBP for 6 h. (C) Percent CD4⁺ T cells secreting IFN-γ; (D) secreting IL-17 and (E) secreting IFN-γ and IL-17 following stimulation with HKBP for 6 h. (F) Percent CD8⁺ T cells secreting IFN-γ among splenocytes following stimulation with HKBP for 6 h. (G) Anti-B. pertussis-specific IgG antibody in serum, (H) anti-B. pertussis-specific IgA antibody in serum of either treated mice with anti-CD71 or isotype control group were measured by ELISA as described in methods. Each point represents data from an individual mouse, representative of three independent experiments. Bar, mean ± one standard error.