Table 1.

Kinetic growth parameters: growth rate μ, biomass yield from substrate Y_X/S, for different P. putida strains

Genotype	WT	pSEVA224‐cscA	pSEVA224‐cscAB	PP_0075::cscA	PP_3398::cscAB
Mediuma	1.36 g/L Glc + 1.5 g/L Fru	3 g/L Sucrose + 15 mg/L Km	3 g/L Sucrose + 15 mg/L Km	3 g/L Sucrose	3 g/L Sucrose
μ [h−1]	0.45 ± 0.02e	0.36 ± 0.04b	0.38 ± 0.01b	0.27 ± 0.05b	0.27 ± 0.01e
Yxsd [g/g]	0.172 ± 0.009b	0.253	0.269	0.210 ± 0.005b	0.218 ± 0.003e
lag phase [h]c	not determined	4.2 ± 1.4	3.4 ± 0.3	not detectable	not detectable

Glc, glucose; Fru, fructose; Km, kanamycin.

^aM9 was used as standard medium, only substrates and additives are noted here.

^bStandard deviations calculated from three biological replicates.

^cDuration of the lag phases were estimated from the intercept of the exponential growth curve with the initial OD₆₀₀, for genomic integrated strains the initial OD was a lot higher which led to no observable lag phase.

^dDuring exponential growth phase.

^eStandard deviations calculated from two biological replicates.