Figure 3.

PrgX binds differently to sequences that have 5 or 10 bp inserted between two operators. EMSA experiments were performed as described in Figure 2. Purified PrgX protein concentrations used in the experiment: lanes 2, 5, 8: 10 nmol L⁻¹; lanes 3, 6, 9: 25 nmol L⁻¹; lanes 4, 7, 10: 100 nmol L⁻¹. PrgX was preincubated with DMF, C or I for 5 min before addition of probes. (a). DNA probe has 5 bp inserted between XBS1 and XBS2 sites. (b). DNA probe has 10 bp inserted between the two binding sites. EMSA experiments were performed as described above