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. 2017 Mar 30;6(4):e00468. doi: 10.1002/mbo3.468

Table 1.

Strains and plasmids

Strains or plasmids Description Source
Strains
 Aeromonas hydrophilaB11 Wild‐type strain (SmR) (Qin et al., 2014)
 AM01~AM102 Mini‐Tn10Km insertion mutant (SmRKmR) Our study
 AM73 (acuC−) AcuC:: mini‐Tn10Km (SmRKmR) Our study
 AC73 (acuC+) AM73 complemented with pACYC184‐AcuC (SmRKmRCmr) Our study
 E. coliSM10 thithrleutonAlacYsupErecARP4 – 2 ‐ Tc:: Mu:: Km (λpir) (Qin et al., 2013)
 E. coliDH5α F, φ 80dlacZΔM15, ΔU169 (lacZYA‐argF), deoR, recA1endA1, hsdR17 (rK ,mK + ), phoA, supE44, λ , thi ‐1, gyrA96, relA1 Takara (Qin et al., 2014)
Plasmids
pMD18 ‐ T Cloning vector (ApR) Takara
pLOF/Km Tnl0‐connected transmit plasmid (KmRApR); (Herrero et al., 1990)
pACYC184 (CmRTcR) afforded by Prof. Nie
pACYC184 ‐acuC Recombination of pACYC184 and AcuC including the promoter and ORF (CmR) Our study