Table 4.
Primers designed for qRT‐PCR
| Gene | Primer | sequence (5′ → 3′) |
|---|---|---|
| acuC | Ac‐F | AGGACGATGCCTACCTCACC |
| Ac‐R | GCGTTTGTTCGCCTCTTCA | |
| cblA | Ad‐F | CCGAGGCGTTCTATGTGCA |
| Ad‐R | TTGGTCAGGTAGCCGGTGAT | |
| aerA | Ae‐F | GGTCTGTGGCGACAAGTATCG |
| Ae‐R | AGAGCAGACAGAGTCGGTATTTCTC | |
| ascV | As‐F | GGGTATTCACCTGCGTTTCA |
| As‐R | GATGTTCATTAGCGACCCACA | |
| hlyA | Hl‐F | CCGCCCAGTCCTTCATCTAT |
| Hl‐R | AGGGTCCGTAGGCTCACATT | |
| ompA | Om‐F | CTCACGATCTGGGTGACTTTG |
| Om‐R | CGCCGTTGATGGACTTGA | |
| ompTS | Pr‐F | AATGGCTCCTTCCCTGATCG |
| Pr‐R | TGGCACCCTGGTTCTCGTAA | |
| vash | Va‐F | AAACTGGCACGGGGAAAGAG |
| Va‐R | GCTTGTAAGGTGAGCGGCATAT | |
| traA | Tr‐F | GTGATGGTCGTCGCCTTTCT |
| Tr‐R | GATAACCTTCTCCGCATTTTCC | |
| pilB | Pi‐F | CTAATGCGAATGCAGCACGTA |
| Pi‐R | CGCTTCAACAGTTCCAACCA | |
| rfaf | Rf‐F | TACCTGGCACTGGCCTATCC |
| Rf‐R | CTCGTCGAGGTGCTTTTGTG | |
| degQ | De‐F | CACCGAGCTTACCTCCGAAAT |
| De‐R | CCGCCTTCTTCAGCGTGAC | |
| flgE | Fe‐F | CCCGCTCAGACATTGGAGAT |
| Fe‐R | GTCGCATTGCTGTAGGTCGC | |
| flgL | Fl‐F | GCCCCAGAACAACAACATCC |
| Fl‐R | GCCGCATCCTCTTTTGACA | |
| 16s | 16s‐F | GGGGAGTACGGTCGCAAGAT |
| 16s‐R | CGCTGGCAAACAAGGATAAGG |