Figure 1.

Real‐time quantification of NO concentration and growth of EHEC in the presence of various NO donors under anaerobic conditions. (a) The NO steady‐state levels in LB broth (pH 7.0) in the presence of various NO donors were measured using an amiNO‐2000 NO electrode at 37°C under anaerobic conditions. Representative NO electrode data are shown (n = 3). (b, c) EHEC strains grown overnight were diluted with LB broth containing various concentrations of NO donor and grown statically for 24 hr at 37°C under anaerobic conditions. The OD ₆₀₀ in culture was measured by spectrophotometer at the indicated times. Data are the means ± standard deviations of values from three experiments. Results are shown for the deleted norV‐type wild EHEC EDL933 (b) and the intact norV‐type wild EHEC K15 (c).