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. 2017 Mar 15;6(4):e00461. doi: 10.1002/mbo3.461

Figure 2.

Figure 2

NO enhances Shiga toxin production and RecA expression in the deleted norV‐type EHEC under anaerobic conditions. EHEC EDL933 grown overnight was diluted with LB broth containing DETA‐NONOate (DETA/NO) and then grown statically at 37°C under anaerobic conditions. (a) EHEC strains were fractionated into the cell‐associated fractions at the indicated times. A 0.2 μg sample of each protein of the cell‐associated fraction was analyzed by Immunoblot analysis using anti‐Stx1 antiserum, anti‐RecA antibody, and anti‐RNA α antibody as an internal control. (b) EHEC strains were fractionated into the cell‐associated fractions at 18 hr. A 0.2 μg sample of each protein of the cell‐associated fraction was analyzed by Immunoblot analysis using anti‐Stx1 antiserum and anti‐RNA α antibody as an internal control. (c) EHEC strains were fractionated into the cell‐associated fractions and the culture supernatant fractions at the indicated times. A 0.2 μg sample of each protein of the cell‐associated fraction was analyzed by Immunoblot analysis using anti‐RecA antibody and anti‐RNA α antibody as an internal control. Each volume, which corresponds to 0.2 μg of protein of the cell‐associated fraction, of the supernatant fraction was analyzed by Immunoblot analysis using anti‐Stx2 antiserum. (d) EHEC strains were fractionated into the cell‐associated fraction and the culture supernatant fractions at 18 hr. A 0.2 μg sample of each protein of the cell‐associated fraction was analyzed by Immunoblot analysis using anti‐RecA antibody and anti‐RNA α antibody as an internal control. Each volume, which corresponds to 0.2 μg of protein of the cell‐associated fraction, of the supernatant fraction was analyzed by Immunoblot analysis using anti‐Stx2 antiserum.